Complex Formation of Cytochrome P450cam with Putidaredoxin
نویسندگان
چکیده
منابع مشابه
The Role of Water Molecules in the Association of Cytochrome P450cam with Putidaredoxin
We have investigated the osmotic pressure dependence of the association between ferric cytochrome P450cam and putidaredoxin (Pdx) to gain an insight into the role of water molecules in the P450cam-reduced Pdx complexation amenable to physiological electron transfer. The association constant was evaluated from the electron transfer rates from reduced Pdx to P450cam. The natural logarithm of the ...
متن کاملCombined QM/MM Studies of Binding Effect of Cytochrome P450cam to Putidaredoxin
Combined QM/MM calculations of an activesite of Cytochrome P450cam have been performed, before and after a binding process of Cytochrome P450cam to Putidaredoxin. The calculations have been carried out for two coordination spheres of the heme active-site of Cytochrome P450cam, namely a 5-coordinated, and a 6coordinated system of this protein having a water molecule as a 6th ligand. An experimen...
متن کاملThe role of water molecules in the association of cytochrome P450cam with putidaredoxin. An osmotic pressure study.
We have investigated the osmotic pressure dependence of the association between ferric cytochrome P450cam and putidaredoxin (Pdx) to gain an insight into the role of water molecules in the P450cam-reduced Pdx complexation amenable to physiological electron transfer. The association constant was evaluated from the electron transfer rates from reduced Pdx to P450cam. The natural logarithm of the ...
متن کاملDetection of a high-barrier conformational change in the active site of cytochrome P450cam upon binding of putidaredoxin.
The orientation of the substrate camphor in the active site of reduced CO-bound cytochrome P450cam (CYP101) as a function of reduced putidaredoxin (Pdxr) addition has been examined by NMR using perdeuterated CYP101 and perdeuterated Pdx as well as isotopically labeled d-camphor. This permits the 1H resonances of CYP101-bound camphor to be observed without interference from the signals of CYP101...
متن کاملCrystal Structure of the Putidaredoxin Reductase- Putidaredoxin Electron Transfer Complex
CRYSTAL STRUCTURE OF THE PUTIDAREDOXIN REDUCTASEPUTIDAREDOXIN ELECTRON TRANSFER COMPLEX Irina F. Sevrioukova, Thomas L. Poulos, and Inna Y. Churbanova⊥, From the Departments of Molecular Biology and Biochemistry, Chemistry and Pharmaceutical Sciences, University of California, Irvine, California 92697-3900 Running title: Crystal structure of the Pdr-Pdx complex Address correspondence to: Irina ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2002
ISSN: 0021-9258
DOI: 10.1074/jbc.m108917200